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(8-9) Thaw at various rates 0 10 20 30 4b- 50 MINUTES Page 305 Cryopreservation of Rat Hepatocytes 311 8 Lower the N flow to purge levels and remove the cot1 from the hqutd nitrogen Defrost the system with the hair dryer until the cot1 and the tubing are at room temperature Turn st ructure N gas flow 9 Charactertzatton of Cultured Hepatocytes Cultures should be followed for 2 wk with media changes and samples taken dally.
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